Insertion junction: LMJ.RY0402.242093_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242093
Chromosome:chromosome 7
Location:945102
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g319150 PTP4 Protein tyrosine phosphatase; (1 of 2) PTHR10367 - MRNA-CAPPING ENZYME intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCGGCCAGCGCGCGTGTGACGGCCCAC
Internal bar code:GCGGGTAAAACCGGCGCGCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:12.1915
LEAP-Seq n confirming:662
LEAP-Seq n nonconfirming:4768
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAAGAACATCAACCACG
Suggested primer 2:CTTGTCGTGTCGTCGATCC