| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242100 |
| Chromosome: | chromosome_9 |
| Location: | 6540455 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g407801 | AKC1 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATACGCATACTTCACACGCTTGCATTCAC |
| Internal bar code: | AACTCTCGATAAGTTAATGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.6121 |
| LEAP-Seq n confirming: | 2568 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGTATCTACAACCCCA |
| Suggested primer 2: | GCACTTGCTTTGCCTTTTTC |