| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242125 |
| Chromosome: | chromosome 3 |
| Location: | 4984631 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180100 | UNG2 | Uracil DNA glycosylase; (1 of 1) 3.2.2.29 - Thymine-DNA glycosylase / Uracil/thymine DNA glycosylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTAACATTGCTTGCGTGGCGCTGGCGGT |
| Internal bar code: | CCCCCTGTGACCCACGAATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCTTAACAACGACGGT |
| Suggested primer 2: | GCGGTATGGTGGAATGGTAG |