| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242168 |
| Chromosome: | chromosome 8 |
| Location: | 3875749 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377900 | (1 of 7) PF05869 - DNA N-6-adenine-methyltransferase (Dam) (Dam) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTTAATTCAGAAGAAGCCCGGTGGGGG |
| Internal bar code: | TGGCTCGCATGCAAATGGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 797 |
| LEAP-Seq percent confirming: | 89.5441 |
| LEAP-Seq n confirming: | 1807 |
| LEAP-Seq n nonconfirming: | 211 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCTCCTGCTCCTCCT |
| Suggested primer 2: | TGTTGTAGCCGGGTATGTGA |