Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.242174 |
Chromosome: | chromosome_10 |
Location: | 1751688 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g430700 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAGTTACTCCGTGCTCCATGAACGCAACC |
Internal bar code: | CAGGCACCCCGTGGGTCTAGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 564 |
LEAP-Seq percent confirming: | 99.3578 |
LEAP-Seq n confirming: | 1083 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTACCGTACTGGGGCTAT |
Suggested primer 2: | CACAGCCTCAGTCAGTCCAA |