| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242196 |
| Chromosome: | chromosome_17 |
| Location: | 1037866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g703600 | OFD1 | Basal body protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTTTCAGCTCCCAGCTTCTTCATCATTGC |
| Internal bar code: | GTGCGCGTATCCGATGAGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 98.7539 |
| LEAP-Seq n confirming: | 317 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAAACTTTGACCGACCG |
| Suggested primer 2: | TTTCCTATTGCATTCCTCCG |