Insertion junction: LMJ.RY0402.242201_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.242201
Chromosome:chromosome 7
Location:4381407
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g341154 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACCACGTTGACAGAATCTTGGTACCCG
Internal bar code:ATTTTTCGTACGGGCGATCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:224
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTCTTGGGAACACACTTT
Suggested primer 2:GCATCTTCCTGCAGTTGTCA