| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242207 |
| Chromosome: | chromosome 2 |
| Location: | 4630006 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101150 | (1 of 2) IPR000104//IPR000210//IPR011333 - Antifreeze protein, type I // BTB/POZ domain // POZ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACGGGGTCGAATGGGTGCGCGGTACGG |
| Internal bar code: | CGGCCGTCGGAAAAAGGGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 97.5535 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCCACCCATCCACTCAC |
| Suggested primer 2: | GCTCTTGCGTAGGACTGACC |