| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242228 |
| Chromosome: | chromosome 9 |
| Location: | 1047400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400750 | AMT5,AMT1Ea,AMT1E | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCATCCTGCAATGGGCGCTAGGCATCAC |
| Internal bar code: | CTCCAGGTAGCATCAAACTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 95.1841 |
| LEAP-Seq n confirming: | 336 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCTGTCGTCATTCTTAT |
| Suggested primer 2: | GGATTCTGACATTGCCGTTT |