| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242245 |
| Chromosome: | chromosome 7 |
| Location: | 4571599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343933 | EBM6 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); Mannan endo-1%252C4-beta-mannosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCTGCTGACCTAGCAGCCACCACGGC |
| Internal bar code: | GCCGGAAGGCACCGTCTGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 99.1542 |
| LEAP-Seq n confirming: | 4689 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCACTGATGCCACCACC |
| Suggested primer 2: | ATCCCACCCCACCTATCTTC |