| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242245 |
| Chromosome: | chromosome 17 |
| Location: | 2744840 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718300 | (1 of 1) K10144 - RING finger and CHY zinc finger domain-containing protein 1 (RCHY1, PIRH2) | CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTGGGTGGGGGCGTGCATGGGTGGGT |
| Internal bar code: | AGGCGGTCGCTACGAAGCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 97.7311 |
| LEAP-Seq n confirming: | 1163 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTTGCTTTTGGGACTCA |
| Suggested primer 2: | GTCGTCGTCGTAAAAGGAGC |