| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242263 |
| Chromosome: | chromosome 16 |
| Location: | 7273395 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683259 | MCP29 | (1 of 1) PTHR24089:SF270 - SOLUTE CARRIER FAMILY 25 MEMBER 48; Mitochondrial substrate carrier protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTCGTGCTGCTTCGTATTGAAGCCCC |
| Internal bar code: | AGCTGCATAATGCAGGATCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 99.2839 |
| LEAP-Seq n confirming: | 5130 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGAAAAACAGGCTCGGG |
| Suggested primer 2: | TGCGACTGATCCTTTCACTG |