| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242302 |
| Chromosome: | chromosome 3 |
| Location: | 8047745 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201350 | (1 of 100) IPR001810 - F-box domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCTACCGAGGAAGGGCAAGGCAAAAG |
| Internal bar code: | GAGTCTTTTTCTTGAGCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 624 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTGAGAAGTCCCCTG |
| Suggested primer 2: | GACCCATGCACAGTTTGTTG |