| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242307 |
| Chromosome: | chromosome 7 |
| Location: | 3279239 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334350 | (1 of 3) 2.7.1.39 - Homoserine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACGTGCCCTCAAACGCACTGCTCTCA |
| Internal bar code: | AGGGTTCTAGCATCTACTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.5209 |
| LEAP-Seq n confirming: | 5401 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGGTGTTTGCTTTAAGTG |
| Suggested primer 2: | TGTTCGTCTGAACTTGCTGG |