Insertion junction: LMJ.RY0402.242329_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.242329
Chromosome:chromosome 5
Location:622184
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g232002 ACX2 Acyl-CoA oxidase/dehydrogenase; (1 of 2) PTHR10909//PTHR10909:SF317 - ELECTRON TRANSPORT OXIDOREDUCTASE // ACYL-COENZYME A OXIDASE 2, PEROXISOMAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACGGCCTGCATCCCATCTGCACCTGTCA
Internal bar code:GGCACGTTCTTCACATAGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:90
LEAP-Seq percent confirming:98.6842
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAATCAACCTTCCGCAAC
Suggested primer 2:GTCCAGTCAGTGTCGCTTGA