Insertion junction: LMJ.RY0402.242331_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242331
Chromosome:chromosome 3
Location:1966722
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g155700 (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAACGGTGGCGCATTTGTCGACTTCAGC
Internal bar code:ATGCACCGAGTTCCTCCTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:219
LEAP-Seq percent confirming:99.4924
LEAP-Seq n confirming:392
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCTTTCAGGTAACCACAT
Suggested primer 2:GATGGCGGTGAACACTACCT