Insertion junction: LMJ.RY0402.242334_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242334
Chromosome:chromosome 2
Location:992461
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g080300 (1 of 5) PTHR10791 - RAG1-ACTIVATING PROTEIN 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACTTCGTGGTGAGGACCGCAAAGGTTCG
Internal bar code:GATCGCTTGGTGCGGCAATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:344
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGAACCACAGCCATCAC
Suggested primer 2:CCACCGACATCATGTGCTAC