| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242381 |
| Chromosome: | chromosome 6 |
| Location: | 3025254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273950 | HFO7 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGTTTCTCAACACCACCCTGACACGT |
| Internal bar code: | AACCTATCGGTGCTACTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 96.4232 |
| LEAP-Seq n confirming: | 647 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGGTGTCCTCGAACAG |
| Suggested primer 2: | CAGGAAGGCCTATGGAATGA |