Insertion junction: LMJ.RY0402.242381_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.242381
Chromosome:chromosome 6
Location:3025254
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g273950 HFO7 (1 of 33) K11254 - histone H4 (H4); Histone H4 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTTGGATGAAGCGGTAGACAGCCAGGTG
Internal bar code:GTGACTCGCTGCTACTTTGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTGGTGTCCTCGAACAG
Suggested primer 2:CAGGAAGGCCTATGGAATGA