| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242403 |
| Chromosome: | chromosome 3 |
| Location: | 1112621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148900 | FMO5 | Flavin-containing monooxygenase; (1 of 3) K00485 - dimethylaniline monooxygenase (N-oxide forming) (FMO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATGGCACTTGACCCGATCACGGCCCGCA |
| Internal bar code: | GGGGTCATAGTAATTTGGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 98.324 |
| LEAP-Seq n confirming: | 352 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGCTGGTCGTGGTACT |
| Suggested primer 2: | TACTGGATAGCGTGCGTCTG |