| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242416 |
| Chromosome: | chromosome 16 |
| Location: | 4255413 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCAGCTTCAGAGGCGCCAGACCGCCTG |
| Internal bar code: | TGTACTGAATGGCCCCTAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1114 |
| LEAP-Seq percent confirming: | 99.1143 |
| LEAP-Seq n confirming: | 3693 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTGGTACACGTTCTCC |
| Suggested primer 2: | TGCAGCTCCAGCACAAGTAG |