| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242464 |
| Chromosome: | chromosome 10 |
| Location: | 1209685 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426350 | predicted protein; (1 of 2) 5.2.1.14 - Beta-carotene isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGTGGAATGGGTGCGGTCGTGGCCAT |
| Internal bar code: | GCATGGTATGGGGGGCAGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 99.2625 |
| LEAP-Seq n confirming: | 673 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGAATCTCCACAAGGAGG |
| Suggested primer 2: | GTTCAGCCTTGTGCTGTTGA |