| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242474 |
| Chromosome: | chromosome 5 |
| Location: | 1104485 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245950 | DRP1 | Dynamin-related GTPase; (1 of 2) PF01031//PF01926//PF02212 - Dynamin central region (Dynamin_M) // 50S ribosome-binding GTPase (MMR_HSR1) // Dynamin GTPase effector domain (GED) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTTTTTCCCGCGCAGAGTCTCCAACC |
| Internal bar code: | GAGAAAGGCGCAGGTCAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCATCTGTGGACACCACG |
| Suggested primer 2: | TGTGATTTCCTGCATGTGGT |