| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242490 |
| Chromosome: | chromosome 8 |
| Location: | 411606 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358578 | (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGCTCCCGGTCTTTATGCCTCTGCTCA |
| Internal bar code: | ATGGACATTAGAATTCGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 74.2284 |
| LEAP-Seq n confirming: | 481 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTCACCTGCAAGCTGTC |
| Suggested primer 2: | AGCTCATGGACAGGACCAAC |