Insertion junction: LMJ.RY0402.242497_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242497
Chromosome:chromosome 11
Location:1109983
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTCTGTGAGCTTGTCAAACGCGGTGAA
Internal bar code:TAAGACTGTACACAGCGCACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:99.5763
LEAP-Seq n confirming:470
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTTCCTCAGATGTGTTCA
Suggested primer 2:ATTATTGGCACCACTCCTGC