| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242515 |
| Chromosome: | chromosome 10 |
| Location: | 3288921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443350 | (1 of 1) PTHR13258//PTHR13258:SF0 - UNCHARACTERIZED // COILED-COIL DOMAIN-CONTAINING PROTEIN 132 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGTGGCAAGCCGAAAGCCCCAGCCCA |
| Internal bar code: | TACGCTCTATGCGACCCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 97.2455 |
| LEAP-Seq n confirming: | 812 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGCATTATTAGGCAAA |
| Suggested primer 2: | AACATGTCCCTGGACCTCAG |