| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242520 |
| Chromosome: | chromosome_14 |
| Location: | 2458536 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g624950 | DHC10,IDA2,DYH1B | Flagellar inner arm dynein 1 heavy chain beta | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCACGAAGAACTACCGCCAGACGGAGTTC |
| Internal bar code: | AGATCGAACACTACTCCCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 351 |
| LEAP-Seq percent confirming: | 99.4328 |
| LEAP-Seq n confirming: | 10518 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGGTAGGTGTGGAGGTA |
| Suggested primer 2: | CACACCCAACCCTTACCATC |