Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.242520 |
Chromosome: | chromosome_17 |
Location: | 3855853 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g728200 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTCCCAGCAACAAGCCAGGGGGGGAAGAG |
Internal bar code: | AGGCTTCGGGCATGCCAGCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 477 |
LEAP-Seq percent confirming: | 96.5268 |
LEAP-Seq n confirming: | 667 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGGAAGAGGCAGTCGATGA |
Suggested primer 2: | CACCAACCATAAGTGCAACG |