Insertion junction: LMJ.RY0402.242529_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.242529
Chromosome:chromosome 6
Location:8166745
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g305250 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGGATACACCCCACGACACAGATGGGTT
Internal bar code:GCGCCTCCCGATGGATGAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:562
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:138
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCCAAATCTCTCGGTGA
Suggested primer 2:AGAAGAGCTTCCAGTGCAGG