Insertion junction: LMJ.RY0402.242542_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.242542
Chromosome:chromosome 7
Location:3024264
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g333400 MMP23 Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGACAAGTGATTTCACTATCTGTATTCC
Internal bar code:CGCAGGTCTACAAACAAGGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:632
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:496
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTGCGACAACCTCATCA
Suggested primer 2:AATTTCCCCAAGTAGCCCAC