| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242548 |
| Chromosome: | chromosome 10 |
| Location: | 6013222 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462850 | (1 of 2) PTHR10283//PTHR10283:SF21 - SOLUTE CARRIER FAMILY 13 MEMBER // ARSENICAL PUMP MEMBRANE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACGTGATGGCTTGCAGCAGGAGCAACA |
| Internal bar code: | GGACGGATAGGGGTGGGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 99.4103 |
| LEAP-Seq n confirming: | 1180 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGAACCTCCTGCTTCAT |
| Suggested primer 2: | TGGTTATGGGCTAGGATTGC |