Insertion junction: LMJ.RY0402.242548_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.242548
Chromosome:chromosome 10
Location:6013224
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g462850 (1 of 2) PTHR10283//PTHR10283:SF21 - SOLUTE CARRIER FAMILY 13 MEMBER // ARSENICAL PUMP MEMBRANE PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGGACATCGGCGTGGCGGTGTCAGGCGA
Internal bar code:GATTGACTCGGCTTATTGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CATGGAACCTCCTGCTTCAT
Suggested primer 2:TGGTTATGGGCTAGGATTGC