| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242576 |
| Chromosome: | chromosome 10 |
| Location: | 1286875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427250 | PRO1,PFN1,PRF1,DIV68 | (1 of 1) K05759 - profilin (PFN); Profilin 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTTAGGGTAGAGATAGAGAGGCATGGA |
| Internal bar code: | TTTCCACATGGGAATATCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 99.3386 |
| LEAP-Seq n confirming: | 4656 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGTTCGATGTTCGGTCC |
| Suggested primer 2: | CGATGTAACACATCATCGGC |