| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.242598 |
| Chromosome: | chromosome 6 |
| Location: | 3023036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273850 | HTB7 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGCTGGACCGTGTCTTCTACCGTCGTT |
| Internal bar code: | GTCCACGGTCTGAGGGCATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 650 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCAGGTAGCGTGCAATG |
| Suggested primer 2: | TACCTTTCCAGTTTGCCCAC |