| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242613 |
| Chromosome: | chromosome 4 |
| Location: | 452599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217923 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGGCCTAGACATTAGAATGCCGCGTTG |
| Internal bar code: | TGGTGTGCCGAGGGAGACTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 91.1302 |
| LEAP-Seq n confirming: | 1274 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCGGCTTGTTAAGATCA |
| Suggested primer 2: | AGCTTGCACCGTATACACCC |