| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242666 |
| Chromosome: | chromosome_13 |
| Location: | 3741449 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589450 | antisense | 5'UTR/CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGACGATTGAGGTTGGCGACTGGCTCTCC |
| Internal bar code: | TGGGTCCCAACACCTTTCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 96.5636 |
| LEAP-Seq n confirming: | 562 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTAGCTGAACTCCACCG |
| Suggested primer 2: | AACATGCCTGGTTGTAAGCC |