| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242673 |
| Chromosome: | chromosome 3 |
| Location: | 4329616 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174950 | TGL7 | (1 of 3) PTHR21493//PTHR21493:SF87 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SN1-SPECIFIC DIACYLGLYCEROL LIPASE BETA; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGTGCGCCCGCGTGAGCAGGCCGACC |
| Internal bar code: | CGAATTCGCGTCAGAATCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 99.7066 |
| LEAP-Seq n confirming: | 16652 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTTCCCTTCCTCATCCT |
| Suggested primer 2: | CTGAGCATCTCCTGACCCTC |