Insertion junction: LMJ.RY0402.242694_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242694
Chromosome:chromosome 8
Location:1934331
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367350 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAATCGGTCGTTGACGTTGCCCGTTGA
Internal bar code:ATCCTCTCATGGTATCCCAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:309
LEAP-Seq percent confirming:98.913
LEAP-Seq n confirming:182
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACACACCGCACACACACAC
Suggested primer 2:ACACAAGCACACACAGGAGC