| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242699 |
| Chromosome: | chromosome 14 |
| Location: | 3386431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630301 | (1 of 1) IPR000104//IPR001890//IPR029058 - Antifreeze protein, type I // RNA-binding, CRM domain // Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGCGGCGGCGGCACGTGACCCGGGCC |
| Internal bar code: | AGCCGCGAACATATTTTATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 99.2095 |
| LEAP-Seq n confirming: | 251 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGGTGGGTGCTGTTTTT |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |