| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.242706 |
| Chromosome: | chromosome 14 |
| Location: | 2593456 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625802 | (1 of 1) K10696 - E3 ubiquitin-protein ligase BRE1 [EC:6.3.2.19] (BRE1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACATGGCGTAAAGCGCGTACGGTGGATC |
| Internal bar code: | GGCGAGCAGCGGACTGGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 99.2674 |
| LEAP-Seq n confirming: | 813 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACACAGTGCTGCGTGTT |
| Suggested primer 2: | TTTGTTGCAGCTTGGTAACG |