| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242722 |
| Chromosome: | chromosome 2 |
| Location: | 6820899 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119100 | CER2 | (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAATGAAGCGACGGAGAGCGGGCGTCTG |
| Internal bar code: | CGCATAGGTCACAGGCACGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 98.1991 |
| LEAP-Seq n confirming: | 1036 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATTCAACCCGCCTGACTA |
| Suggested primer 2: | CACATACACACCTTGTCGCC |