Insertion junction: LMJ.RY0402.242740_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.242740
Chromosome:chromosome 1
Location:5844443
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g041752 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCTTCCCCGCTTCAGATGATGATGACC
Internal bar code:TTGGGAAAGCCAGTTTCAGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:757
LEAP-Seq percent confirming:99.0279
LEAP-Seq n confirming:815
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTGTTACGGCCAGAGA
Suggested primer 2:CGAAGCTTGGGAAGTAGTGC