Insertion junction: LMJ.RY0402.242744_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.242744
Chromosome:chromosome 16
Location:6823967
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g675076 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAGGGGCTCCAAGGTGAACACGGAGCGC
Internal bar code:GTCGGTTACGGTCCGACACGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:295
LEAP-Seq percent confirming:98.9362
LEAP-Seq n confirming:279
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATGAGCACAGATCGAGGG
Suggested primer 2:GTCCATGTCCACATCAGCAG