| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.242744 |
| Chromosome: | chromosome 16 |
| Location: | 6823967 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675076 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGGGGCTCCAAGGTGAACACGGAGCGC |
| Internal bar code: | GTCGGTTACGGTCCGACACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 98.9362 |
| LEAP-Seq n confirming: | 279 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAGCACAGATCGAGGG |
| Suggested primer 2: | GTCCATGTCCACATCAGCAG |