| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242759 |
| Chromosome: | chromosome 1 |
| Location: | 2142547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011630 | (1 of 1) IPR001283//IPR002413//IPR003882//IPR014044 - Cysteine-rich secretory protein, allergen V5/Tpx-1-related // Ves allergen // Pistil-specific extensin-like protein // CAP domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGCCTACGCAACCCCTTTGCCTATCAA |
| Internal bar code: | ATGGGCACTAAGAGAAGTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.8911 |
| LEAP-Seq n confirming: | 917 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATATTACGCAGCAGCAG |
| Suggested primer 2: | ATTTGACAACTGGGCTGTCC |