Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.242799 |
Chromosome: | chromosome_10 |
Location: | 3832420 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g447300 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACTTCGCCAATCGGTGGTAGGACCAAGGCA |
Internal bar code: | TCAACAGGTTGGCCGCCCATGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 599 |
LEAP-Seq percent confirming: | 98.6245 |
LEAP-Seq n confirming: | 717 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGAGAGGACAAGCATACC |
Suggested primer 2: | ACGCTGTCTGGATGCCTAGT |