Insertion junction: LMJ.RY0402.242821_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.242821
Chromosome:chromosome_13
Location:4603349
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):ACCACAACTCCCACGCCACATGTCACACTC
Internal bar code:CCGTAATCGGGGACACCCAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:532
LEAP-Seq percent confirming:98.6279
LEAP-Seq n confirming:2372
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AAACGTGTCAGGCAACACTG
Suggested primer 2:AACCGTGGGTGTATCCATGT