Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.242855 |
Chromosome: | chromosome_9 |
Location: | 7033260 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g411200 | TEF5 | Rieske [2Fe-2S] domain containing protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCCACTTCCTCCCTTCCTCCCACTCCCGC |
Internal bar code: | GCGGGCTCCGGCCGGGGTACTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 388 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 28 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGCGTCATGTCCATGTTT |
Suggested primer 2: | AGGGGAAGAGGAAATGAGGA |