| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242876 |
| Chromosome: | chromosome 12 |
| Location: | 3504624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512950 | CIT1 | (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACTTAGTGGCGGGGGATGATATTAAA |
| Internal bar code: | ACCTGTTTATCCAATGGTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 99.3965 |
| LEAP-Seq n confirming: | 7082 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTAGCCCTATCCCCAT |
| Suggested primer 2: | TCTACCTCGGTGAAACGTCC |