Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.242884 |
Chromosome: | chromosome_2 |
Location: | 6240620 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g114100 | PDE22 | 3\',5\'-cyclic-nucleotide phosphodiesterase | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCTAGTCGCTTTGAATCCGGCTTGACGCG |
Internal bar code: | TTCGGGGACGATGGTCAGGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 638 |
LEAP-Seq percent confirming: | 99.6956 |
LEAP-Seq n confirming: | 655 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCGAACTCTGGTGTTTGAG |
Suggested primer 2: | ATGACGGTGCTTGGGTAGAC |