Insertion junction: LMJ.RY0402.242917_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.242917
Chromosome:chromosome 16
Location:2952643
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g664150 PDE18 (1 of 3) 3.1.4.17//3.1.4.53 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase // 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAGGTGTTGATGACCGTCTGCCTACGGG
Internal bar code:TGCACTTGGTCTGCGACAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:113
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGTGGCTCTAGCATGTCG
Suggested primer 2:CAGTTGCCGTATATGCATGG